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Darktable edits your images non-destructively all the way through its pipeline. Your original image is never modified! Professional color management features ensure fidelity all the way to print and GPU accelerated processing means you can speed up your workflow using your video card. Mac Pro (Late 2013 and later), MacBook Pro (Late 2013 and later), MacBook Air (Mid 2013 and later), Mac mini (Mid 2014 and later), iMac (Mid 2014 and later), MacBook (Early 2015 and later), iMac Pro (Late 2017) 17 May 2021: Safari 14.1: macOS Catalina and macOS Mojave: 04 May 2021: macOS Big Sur 11.3.1: macOS Big Sur: 03 May 2021: iOS 14.5.1.

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Tannic Acid, Reagent, A.C.S., EM Grade

Search Catalog Search. Shop Categories Back. Shop categories. 12' x 5.9' x 7.4' (1) 12' x 5' x 0.5' (1) 12' x 50' (1) 12' x 5ft (3) 12' x 6.25' (1.

C76H52O46 F.W. 1701.28 CAS #1401-55-4

RT21700100 g13.00Add to Cart

Tannic Acid Solutions

Please see listing in Reagents for Histology.

Technovit® Glycol Methacrylate Embedding Kits

Please see listing in Embedding Media Kits.

TMB, 3,3,5,5-Tetramethyl Benzidine

C16H20N2 F.W.240.35 CAS #54827-17-7
m.p. 166-169°C
Assay >98%
A non-carcinogenic substitute for DAB and DAB-HCl. Used as a sensitive and specific reagent for the detection of blood.

  • Standefer, J.C., Vanderjagt, D. (1977) Assay of Hemoglobin. Clin. Chem. 23, 749.
  • Boss, E.S., et al., (1981). Assay of Peroxidases. J. of Immunoassay. 2,187.
  • Holland, V.R., et al., (1974) Tetrahedron, 30, 3299.
0-5°C130701 g36.00Add to Cart

TMB-HCl, Dihydrochloride TMB

C16H20N2·2HCl F.W. 313.30 CAS #64285-73-0
m.p. >300°C
Assay >98% (Cl)

0-5°C13074TMB-HCl1 gm89.00Add to Cart

Also available in tablet form. 1 mg/ tablet, ready to use.

0-5°C13075TMB-HCl tablets10/bottle39.50Add to Cart
0-5°C13076TMB-HCl tablets50/bottle178.00Add to Cart

(TMA), Tetramethylene Glycol

(1,4-Butanediol dimethacrylate)
[H2C=C9CH3)CO2CH2CH2] 2 F.W. 226.68
CAS #2082-81-7
Specific Gravity (H2O=1) : 1.023
b.p. @4 mmHg 132-134°C
Inhibited with 200 ppm hydroquinone monomethyl ether.

RT21750100 ml18.00Add to Cart

TMS, Tetramethylsilane, 99.9%

Si(CH3)4 M.W. 88.22
b.p. 26.6-26.7°C
Flashpoint: 28°C

An organo-silicon compound tetramethylsilane (TMS) is used as an internal standard for nuclear magnetic resonance (NMR). It is chemically inert and has a boiling point of 26.6°C which is lower than acetone (53.6°C), and other highly volatile liquids, such as diethyl ether (34.5°C) and 1,2-epoxy propane (36.4°C). It is soluble in most organic solvents but insoluble in water. All of these properties make TMS an ideal solvent for tissue drying, which preserves excellent tissue surface details. Dey, Sudip. Et al. (1989). A new rapid method of air-drying for scanning electron microscopy using tetramethylsilane. J. of Microscopy, Vol 156, pp. 259-261.

RT21760100 ml185.00Add to Cart

(TC-NBT), Thiocarbamyl Nitro-Blue Tetrazolium

(2,2'-Di[p-nitrophenyl]-5,5'-di[p-thiocarbamylphenyl] -3,3'-[3,3'-dimethoxy-4,4'-biphenylene]ditetrazolium chloride)
C42H32Cl2N12O6S2 F.W. 935.8 CAS #36889-43-7

RT21800100 mg11.00Add to Cart

Nitro-Blue Tetrazolium

Please see Nitro-Blue Tetrazolium.

(TCH), Thiocarbohydrazide, Highest Purity

H2NNHCSNHNH2 F.W. 106.15 CAS #2231-57-4
b.p. 171°C (decomposed)
Osmiophilic reagent.

An indefinite shelf life due to a special purification process.

  • Seligman A.M. et al (1965).Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH) or thiosemicarbazide (TSC) and osmium tetroxide. J. Histochem. Cytochem., 13:629
  • Seligman, A.M. et al (1966). A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide fixed tissue with osmiophilic thiocarbohydrazide (TCH). J. Cell Biol., 30:424
  • Thiery, J.P. (1967). Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J. Microsc., 6:987
  • Lo, H.K. et al (1987). A modified periodic acid-thiocarbo-hydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. J. Histochem. Cytochem., 35:393
  • Neiss, W.F. (1988). Enhancement of the periodic acid-Schiff (PAS) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) reaction in LR White sections. Histochemistry, 88:603
  • Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science, N.Y., 146:1039
  • Derenzini, M., et al (1986). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glyco-conjugates at the molecular level in situ. J. Histochem. Cytochem.,34:1161
RT219005 g94.00Add to Cart

Thiosemicarbazide, Reagent, A.C.S.

NH2CSNHNH2 F.W. 91.14 CAS #79-19-6
m.p. 181-183°C
Assay >99.0%
References for use-see Thiocarbohydrazide

RT2200025 g12.00Add to Cart

Tissue Marking Dyes

Please see listing in General Supplies for Histology.

Toluene Reagent, A.C.S.

C6H5CH3 FW 92.14 CAS #108-88-3
Specific Gravity (H2O=1): 0.866
b.p. 110-111°C (230-222°F)
Assay. 99.9%

Specifications:

Color (A.P.H.A.)10
Residue after evaporation0.001%
Subs. darkened by H2SO4To pass test
Sulfur compound (as S)0.003%
Water0.03%
RT22030450 ml10.50Add to Cart
RT220324x 450 ml38.00Add to Cart

Toluidine Blue 0, Certified, C.N. #DcU-10

(Basic blue; Methylene Blue T50 or T Extra)
C15H16N3SCl FW 305.83 CAS #92-31-9
Dye Content: approx. 85% min.
Solubility: 3.25% Water; 1.75% Alcohol; 3.5% Cellosolve™; 5.5% Glycol; 0.0% Xylene
*-A general stain for epoxy thick sections. Barajas et al.(1981) Identification of renal neuroeffector junctions by electron microscopy of reembedded light microscopic autoradiograms of semithin sections. J. Ultrastruct. Res. 77: 379.Campbell, R.D., And Hermans, C.O. (1972). A rapid method for resectioning 0.5 4.0 micron epoxy sections for electron microscopy. Stain Technol. 47:115.

RT2205050 g36.00Add to Cart

Technical Tip

Tricresyl Phosphate

(Tritolyl Phosphate)
(CH3C6H4O)3PO F.W. 368.37 CAS #1330-78-5
Specific Gravity (H2O=1): 1.16
A mixture of isomeric Tritolyl Phosphates, usually excluding the very toxic group ortho-isomer. Used to prepare benzoyl peroxide paste, which acts as a catalyst for water miscible resins.

RT22150225 ml22.00Add to Cart

2,4,6-Tri(Dimethylaminomethyl)-Phenol

Please see DMP-30.

RT1360025 ml4.00Add to Cart

2,4,6-Trinitrophenol

Please see Picric Acid.

RT19550100 g94.00Add to Cart

Tris (Hydroxymethyl) Aminomethane,Free-base, Reagent Grade

White crystalline powder
C4H11NO3 F.W. 121.14 CAS #77-86-1
A 40% (w/v) aqueous solution. It is clear and colorless.
Assay >99.9%
Water .<0.2%
Heavy Metals.-2ppm

RT11700Tris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade100 g19.00Add to Cart
RT11720Tris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade500 g72.00Add to Cart

Tris, (hydroxymethyl) aminomethane maleate

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Tris Maleate Buffer 0.2M pH range 6.4-8.0
2-8°C11730Tris Maleate Buffer 0.2M pH range 6.4-8.0250 ml23.00Add to Cart
2-8°C11740Tris Maleate Buffer 0.2M pH range 6.4-8.0500 ml30.00Add to Cart
2-8°C11740-1LTris Maleate Buffer 0.2M pH range 6.4-8.01 L50.00Add to Cart
2-8°C11740-1GTris Maleate Buffer 0.2M pH range 6.4-8.01 Gal.175.00Add to Cart
Tris Buffer 0.2M pH 8
RT11730-05Tris Buffer 0.2M pH 8.0250 ml23.00Add to Cart
RT11730-06Tris Buffer 0.2M pH 8.0500 ml30.00Add to Cart
RT11730-06-1LTris Buffer 0.2M pH 8.01 L50.00Add to Cart
RT11730-06-1GTris Buffer 0.2M pH 8.01 Gal.175.00Add to Cart
Tris Buffered Saline pH 7.4
2-8°C11730-08Tris Buffered Saline pH 7.4250 ml23.00Add to Cart
2-8°C11730-09Tris Buffered Saline pH 7.4500 ml30.00Add to Cart
2-8°C11730-09-1LTris Buffered Saline pH 7.41 L50.00Add to Cart
2-8°C11730-09-1GTris Buffered Saline pH 7.41 Gal.175.00Add to Cart
Tris HCL Buffer 1M pH 6-8
2-8°C11730-12Tris HCL Buffer 1M pH range 6-8250 ml23.00Add to Cart
2-8°C11730-15Tris HCL Buffer 1M pH range 6-8500 ml30.00Add to Cart
2-8°C11730-15-1LTris HCL Buffer 1M pH range 6-81 L50.00Add to Cart
2-8°C11730-15-1GTris HCL Buffer 1M pH range 6-81 Gal.175.00Add to Cart
Tris HCL Buffer pH range 7.2 - 9.0
2-8°C11730-20Tris HCL Buffer pH range 7.2-9.0250 ml23.00Add to Cart
2-8°C11730-25Tris HCL Buffer pH range 7.2-9.0500 ml30.00Add to Cart
2-8°C11730-25-1LTris HCL Buffer pH range 7.2-9.01 L50.00Add to Cart
2-8°C11730-25-1GTris HCL Buffer pH range 7.2-9.01 Gal.175.00Add to Cart

Tris Buffer pH 10.0 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution:
Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.

2-8°C64142-11Tris Buffer pH 10.0100 ml75.00Add to Cart
2-8°C64142-12Tris Buffer pH 10.01000 ml125.00Add to Cart
2-8°C64142-12-1GTris Buffer pH 10.01 Gal.350.00Add to Cart

Triton™ X-100

(Polyethylene glycol tert-octylphenyl ether)
CAS #9002-93-1
Specific Gravity (H2O=1): 1.065

RT22140100 ml12.00Add to Cart
RT22142500 ml46.00Add to Cart

Triton™ X-100, 1% Aqueous Solution

Prepared 1% Triton X-100 in Deionized-Distilled water. Used for whole-cell preparations. Marek, L.F., and Kelly, R.O. (1983). A simple technique for the visualization of whole mount cytoskeletons with transmission electron microscopy. Anat. Rec. 207, 365. Pudney, J., and Singer, R.H. (1980). Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with Triton X-100. Tissue cell 12, 595.

RT22145500 ml42.00Add to Cart

Triton™ X-100, 0.08% Aqueous Solution

A prepared solution for the cleaning of diamond knives.

RT22146500 ml40.00Add to Cart

Trypsin 1:100, Powder

CAS #9002-07-7
Extract from Porcine Pancreas.
Activity >100 NF units/mg
Used for whole cell preparations. (see references in Triton™ X-100)

RT2220025 g22.00Add to Cart

Trypsin Reagent

DownloadDescription: Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes:
Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml
Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml
2-8°C64142-01Trypsin Kit Smallkit75.00Add to Cart
2-8°C64142-02Trypsin Kit Largekit145.00Add to Cart

12-Tungstosilicic Acid

(Silicotungstic acid)
H4[SiO4.(W3O9)4]·xH2O M.W. 2878.29+aq CAS #11130-20-4

RT2224025 g66.00Add to Cart

Tyrode Solution

Ingredients: NaCl; KCl; CaCl2.6H2O; MgCl2.6H2O; NaHCO3; NaH2PO4; Glucose; and Distilled water.

Ordering Information:

RT11760-05Tyrode Solution500 ml26.00Add to Cart
RT11760-10Tyrode Solution1 L36.00Add to Cart

Uranyl Acetate, Reagent, A.C.S.

UO2(OCOCH3)2·2H2O F.W. 424.14
CAS #541-09-3
Assay 98.0-102.0%
Specifications:

Insoluble Matters0.01
Chloride0.003%
Sulfate0.01%
Alkalies (as SO4)0.05%
Heavy Metals0.002%
Iron0.001%

A universal EM stain for thin sections, en-block staining, and negative staining.

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RT2240025 g160.00Add to Cart

UranyLess

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'I am so happy. I read on your web site about UranyLESS and I wanted you to know that I think it did a spectacular job on this sample!' - Debra Townley, Baylor College of Medicine

Substitute for Uranyl Acetate

EMS is proud to introduce UranyLess, a new contrast stain solution for TEM, for all of your negative staining applications. It is an amazing substitute for Uranyl Acetate with similar results.

After only a minute of contact, UranyLess' fast-acting, non-radioactive lanthanide mix is finished staining your sections or deposits (see protocols below). If needed, lead citrate is recommended to increase the contrast. TIP: To prevent precipitates, use room temperature distilled water.

UranyLess's pH level is about 6.8-7. The 30 ml airless bottle will stain approximately 1500 grids. The airless bottle increases shelf life, eliminates CO2 contamination, and produces less waste - the solution pumps out in perfect amounts without leaking or spilling. UranyLess is also available in a larger amount for use in automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.

UranyLess has been tested on many biological tissue (animal and plant): intestine, skeletal and cardiac muscle, liver, kidney, adrenal gland, nerve, cell culture, plant tissue, and also on negative staining of bacteriophage, bacteria, and polymers. UranyLess is ideal because of its ability to stain any kind of material and results are reproducible. To see the effectiveness of Uranyless, please visit the UranyLess Image Gallery.

Lead Citrate 3%

UranyLess has a strong contrasting power, however, we recommend a Lead Citrate counterstain to enhance the contrast. You can follow the protocol of the UranyLess / Lead Citrate double contrast by watching the video. EMS recommends Lead Citrate 3%, ready to use. The special pump delivers the product without letting in air, thus preserving the solution and preventing CO2 dissolution. It is convenient to use because it performs in any position, even upside down. Comes in either a 30 ml Airless bottle or a 30 ml Airless Syringe. The Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100

References:

'Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules.' Benmeradi N1,2, Payre B2 and Goodman SL3.

  1. Delta Microscopies, 22 bis, route de Saint Ybars, 31190 Mauressac, France
  2. Université Toulouse, CMEAB Faculté Medecine, 118 route Narbonne, 31062, Toulouse, France
  3. Microscopy Innovations LLC, 213 Air Park Rd, Suite 101, Marshfield, WI, 54449, USA

'C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle.' Nature Communications. Published 23 Apr 2015. Sandrine Floriot, all.

RT22409UranyLess EM Stain*30 ml52.00Add to Cart
RT22409-20UranyLess EM Stain200 ml250.00Add to Cart
RT22410Lead Citrate 3%, Ready to use*30 ml52.00Add to Cart
RT22410-01Lead Citrate Reynolds Stain 3%, Ready to use syringe**30 ml56.00Add to Cart
* in airless bottle
** Syringe is meant to be used exclusively with the RMC TEM Stainer QG3100

UAR-EMS Uranyl Acetate Replacement Stain

A non-radioactive substitute for uranyl acetate with comparable results. Avoids the tedious authorization, shipping and disposal issues involving uranyl acetate without sacrificing performance. Two lanthanide salts, samarium and gadolinium triacetate, effectively used for staining plastic-embedded animal and plant tissues. Also for negative staining of macromolecules. Not recommended as a fixative.

RT22405UAR-EMS Stain25 ml70.00Add to Cart

Platinum Blue, an EM Stain

IBI Blue Platinum Stain Pt4N8H6O24C20

An EM stain that may have the ability to replace Uranyl Acetate in some instances. If you have issues with having Uranium salts that are radioactive (depleted or not) in the lab this may be the answer for you. An alternative for staining TEM thin sections

RT22407Platinum Blue1 ml250.00Add to Cart

Uranyl Acetate Solution

We are now offering Uranyl Acetate pre-made and ready to use solution for making your work easier. The following percentages are available off the shelf (1, 2, 3, 4). Other percentages are available upon request.

RT22400-11% Uranyl Acetate Solution100 ml39.00Add to Cart
RT22400-22% Uranyl Acetate Solution100 ml45.00Add to Cart
RT22400-33% Uranyl Acetate Solution100 ml64.00Add to Cart
RT22400-44% Uranyl Acetate Solution100 ml76.00Add to Cart

Uranyl Formate

UO2(HCO2)2·H2O

F.W. 378.08
CAS #16984-59-1
For ultra fine grain structure, our Uranyl Formate offers amazing advancement in negative staining for microscopy.

Specifications:

Purity99.9-100%
Specific Gravity3.695
AppearanceYellow Crystals
Melting Point110°C

Ordering Information:

RT22450Uranyl Formate1 gram102.00Add to Cart
RT22451Uranyl Formate5 grams485.00Add to Cart

Uranyl Magnesium Acetate

UO2Mg(CH3COO)4

F.W.530.0

CAS # 20596-93-4

Used as a substitute for uranyl acetate in either double-staining techniques and also as a solo stain.

RT22500Uranyl Magnesium Acetate5 grams216.00Add to Cart
RT22501Uranyl Magnesium Acetate1 gram90.00Add to Cart

Uranyl Nitrate, Hexahydrate

Reagent Grade, A.C.S.
UO2(NO3)2·6H2O

F.W. 502.13

CAS #13520-83-7

Specifications:

Purity99.9-100%
Density2.807 g/cm3
Solubility~66g/100g H2O
Melting Point60°C
Boiling Point118°C
Insoluble Matter<0.005%
Chloride (Cl)<0.002%
Sulfate (SO4)<0.005%
Alkalies % Alkaline Earths (Sulfates)<0.1%
Heavy Metals (as Pb)<0.002%
Iron (Fe)<0.002%
Substances Reducing Permanganate (as UIV)<0.06%

A Universal EM Negative Stain used for viruses. In tissue samples it stabilizes nucleic acids and cell membranes. These solutions are more stable than uranyl acetate and they react primarily with negatively charged groups in the absence of phosphate ions.

RT22600Uranyl Nitrate5 grams120.00Add to Cart

Uranyl Oxalate

(COO)2 UO2 ·.3H2O

M.W.412.2

To adjust the pH of Uranyl compounds as well as a negative stain for electron microscopy.

RT22510Uranyl Oxalate1 gram225.00Add to Cart

Uranyl Zinc Acetate

ZnUO2(CH3COO)4

CAS # 10138-94-0

F.W. 571.59

Used as a laboratory reagent in the determination of sodium concentrations of solutions.

RT22610Uranyl Zinc Acetate5 grams216.00Add to Cart

Technical Tips:

Avoiding Contamination of Ultra-Thin Sections
Removal of Lead Citrate and Uranyl Acetate Precipitates on LR White Sections

Van Gieson's Solution

To use with Weigert's Iodine or Weigert's Iron as a connective tissue stain.

RT26046-05Van Gieson's Solution500 ml36.00Add to Cart
RT26046-06Van Gieson's Solution1 L62.00Add to Cart

Water Deionized, Reagent Grade A.C.S.

H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
Coliform negative

RT22800-01Deionized Water1 gal12.00Add to Cart
RT22800-05Deionized Water5 gal50.00Add to Cart

Weigert's Iodine Solution

A histological stain. Demonstration of connective tissue is useful in cases of emphysema, arteriosclerosis and other vascular diseases

RT26045-05Weigert's Iodine Solution500 ml39.00Add to Cart
RT26045-06Weigert's Iodine Solution1 L69.00Add to Cart

Weigert's Iron Hematoxylin

Stain selectivity for nuclei, fungal, and general applications. Ready-to-use.

RT26044-05Weigert's Iron Hematoxylin A500 ml44.00Add to Cart
RT26044-06Weigert's Iron Hematoxylin A1 L78.00Add to Cart
RT26044-15Weigert's Iron Hematoxylin B500 ml29.50Add to Cart
RT26044-16Weigert's Iron Hematoxylin B1 L52.00Add to Cart

Wright Stain, Certified #DcWr-38

CAS #68988-92-1
Solubility: 0.091% Water; 0.23% Alcohol

RT2272025 g32.00Add to Cart

Wright Staining Solution

For use as a blood stain. Ready-to-use.

RT26060-05Wright Stain Solution500 ml37.00Add to Cart
RT26060-06Wright Stain Solution1 L68.00Add to Cart

Xylenes, Reagent, A.C.S.

(Xylol, Dimethylbenzene)
C6H4(CH3)2 FW 106.27 CAS #1330-20-7
Specific Gravity (H2O=1): 0.865
b.p. 136-140°C
This reagent is a mixture of isomers (ortho, meta, and para) and may contain Ethylbenzene.

Specifications:

Color (A.P.H.A.)10
Subs. darkened by H2SO4To pass test
Sulfur Compounds (as S)0.003%
Water0.05%
Residue after evaporation0.002%
RT23400450 ml8.00Add to Cart

Xylene: Peanut oil, 2:1

RT23402Xylene:Peanut oil, 2:1500 ml69.00Add to Cart

Xylene Substitute

A xylene substitute for use in histology and cytology. It is safer and a more preferable alternative to xylene. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives.

Properties

  • Minimal tissue shrinkage
  • Dries slowly with no residue
  • Less toxic than xylene
  • Soluble with alcohol, paraffin embedding media, and mounting media

It can be used in all procedures that require xylene. Biodegradable.

RT23410-01Xylene Substitute1 gal112.00Add to Cart
RT23410-04Xylene Substitute4x 1 gal420.00Add to Cart
RT23410-05Xylene Substitute5 gal450.00Add to Cart

SlideBrite™ Xylene Substitute

The safest choice for a xylene alternative available today

Reduce your risks & enhance your personnel safety with 'The Original' high quality performance clearing agent. It performs equal-to or better than Xylene, yet smells like water! A non-hazardous clearing agent, it is non-flammable and odorless, with no vapor disguising additives.

Properties

  • Enhances nuclear detail
  • Fast slide drying time
  • Gentle, yet very effective deparaffinization
  • No tissue shrinkage or morphology change
  • No brittle tissue subsequent to tissue processing
RT23401-01SlideBrite™ Xylene Substitute1 gal50.00Add to Cart
RT23401-04SlideBrite™ Xylene Substitute4 x 1 gal190.00Add to Cart

Histosol™ Xylene Substitute

Histosol™ is the original high flash point (114°F TCC) histological clearing agent. It is intended to be used as a replacement for xylene where the hazards associated with aromatic hydrocarbon vapors are to be reduced. Museum-quality tissue slides can be prepared with Hitosol without change in protocol or procedure. Histosol is manufactured from petrochemical products and is miscible in all proportions with ethanol, isopropanol, and t-butanol. It is also miscible with all paraffin-based tissue embedding media and all permanent mounting materials.

RT23405-01Histosol™ Xylene Substitute1 gal68.00Add to Cart
RT23405-04Histosol™ Xylene Substitute4 x 1 gal225.00Add to Cart

Hemo-De® Xylene Substitute

An intensive study from 1992 throughout 2000 has shown that Hemo-De® (d-Limonene) a terpene-based chemical with a pleasant citrus fragrance, was able to replace many of the clinical laboratories toxic reagents, such as carbol-xylene, xylene, ethyl acetate and formalin.

In Parasitology – Hemo-De replaces ethyl acetate in the concentration procedure; Hemo-De replaces carbol-xylene, xylene and formalin in the trichrome procedure.

In Histology – Hemo-De replaces xylene in most all procedures. Hem-De is a superior solvent and Clearing agent that can be used in all tissue processing, deparaffiniating and slide preparation.

Hemo-De Features

  • Soluble with alcohol and mounting media
  • Biodegradable, non-corrosive, non-flammable (Combustible)
  • Contains no benzene and no toluene
  • Low toxicity levels
  • Minimal tissue shrinkage
  • Reasonably fast drying and leaves no residue

Hemo-De Application

In Research and Medical – Hematology, Cytology, Pathology, Microbiology, Histology, Pathology, Hospital

In Mechanical Applications – Cleaning of machine parts, cleaning tools, engines etc.

In Electronic Applications – Circuits boards, electronic components, tools.

General Applications – Clean metal and glass surfaces. Dilute with water can be used to clean all plastic surfaces. Removal of adhesives, glues, tars…

Hemo-De Information

Chemical name:d-Limonene (Terpene Hydrocarbon, n.o.s.)
Composition:d-Limonene, CAS [5989-27-5]98.0%
Butylated Hydroxyanisolve (BHA), CAS
[25013-16-5]…….<0.024%
Flash Point (TCC):121°F (49.4°C)
Boiling Point:349°F (176°C) @ 760mmHg
Freezing Point:-74°C
Shipping:Terpene Hydrocarbons, n.o.s. ID No.
04858003, Flammable Liquid, Group III

References

  1. W.E. Aldeen and D. Hale. 'Use of Hem-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites' J. of Clinical Microbiology, July 1992, 1893- 1895, Vol. 30, No. 7
  2. Metzger, Zvi DMD et al. 'Gutta-Percha Softening: 'Hem-De' as a Xylene Substitute' J. of Endodontics'. 26(7):385-388, July 2000.
  3. Devon C. Hale, MD; et al. 'Use of a Single Slide Trichrome- Stained Concentrate for the detection of intestinal Parasites Stained Concentration Procedure for Ova and Parasites' American Journal of Clinical Pathology, Vol. 106, N0. 2. Aug. 1996.
  4. J. M. Miller et al. 'Biodegradable, effective substitute for xylene in the Ehrlich indole procedure'. J. of Clinical Microbiology, Aug. 1994, 2028-2030, Vol 32, No 8.
RT23412-01Xylene Substitute – Hemo-De®1 gal120.00Add to Cart
RT23412-04Xylene Substitute – Hemo-De®4 x 1 gal450.00Add to Cart
RT23412-05Xylene Substitute – Hemo-De®5 gal469.00Add to Cart

Hemo-De® is a product of Scientific Solvents.

Histo-Clear®/Histo-Clear II®

Histo-Clear® (Distilled essential oils – food grade) and Histo-Clear II® (A mixture of Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.

RT64110-01Histo-Clear®1 L34.00Add to Cart
RT64110-04Histo-Clear®1 gal105.00Add to Cart
RT64110-10Histo-Clear®5 gal450.00Add to Cart
RT64111-01Histo-Clear II®1 L22.00Add to Cart
RT64111-04Histo-Clear II®1 gal80.00Add to Cart
RT64111-10Histo-Clear II®5 gal360.00Add to Cart

Z-Fix Concentrate

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Concentrate for buffered aqueous zinc formalin

Zinc formalin fixatives improve general morphology and immunohistochemical staining and can reduce or eliminate the need for antigen recovery procedures while preserving immunoreactivity for months.

  • Compatible with all tissue processors
  • May be used in place of neutral buffered formalin
  • Easy to dilute with water
  • Make 5 gallons of fixative using just one gallon of concentrate
RT64125-01Z-Fix Concentrate, 1 galeach78.00Add to Cart

Z6040 Embedding Primer

This unique primer affords you a better adhesion between your resin and specimen that you are embedding and prevents the sample from pulling away from the resin during sectioning.

RT50440-10Z6040 Primer100 ml44.00Add to Cart

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